Journal: Cell Reports Medicine

Article Title: Exercise-induced crosstalk between immune cells and adipocytes in humans: Role of oncostatin-M

doi: 10.1016/j.xcrm.2023.101348

Figure Lengend Snippet:

Article Snippet: GDF15 , Bio-Techne LTD , 9279-GD-050.

Techniques: In Vitro, Recombinant, Plasmid Preparation, Fluorescence, Blocking Assay, Protease Inhibitor, Enzyme-linked Immunosorbent Assay, Bicinchoninic Acid Protein Assay, Multiplex Assay, Software, Real-time Polymerase Chain Reaction, Microscopy

Journal: Cell reports

Article Title: Housing mice near vs. below thermoneutrality affects drug-induced weight loss but does not improve prediction of efficacy in humans

doi: 10.1016/j.celrep.2024.114501

Figure Lengend Snippet: Weight loss efficacy in DIO mice at 22°C and 30°C and in humans living with obesity

Article Snippet: Long acting GDF15 , Novo Nordisk A/S , Cat#NNC-0247-0001-0880.

Techniques:

Journal: Cell reports

Article Title: Housing mice near vs. below thermoneutrality affects drug-induced weight loss but does not improve prediction of efficacy in humans

doi: 10.1016/j.celrep.2024.114501

Figure Lengend Snippet: Effects on TEE and activity are comparable. (A) Body weight during treatment of male DIO mice with LA-GDF15 (1 nmol/kg s.c. daily). (B) Body weight at day of transfer to the indirect calorimetry system (day −8) and at the end of treatment (day 21). (C and D) Fat mass and fat-free mass at days −8 and 21. (E) TEE during treatment. (F) Change in TEE vs. change in body weight. Changes were calculated by subtracting the values on the last day of treatment from the day before the treatment started. (G) RER (VCO 2 /VO 2 ). (H) Physical activity. Data are mean ± SEM. Biological replicates: n = 8/group. Technical replicates: n = 1. p values compare the difference between days −8 and 21 by two-way ANOVA with uncorrected Fisher’s LSD post hoc testing within Ta or drug treatment. Color scheme: light blue, 22°C vehicle; pink, 30°C vehicle; dark blue, 22°C LA-GDF15; and red, 30°C LA-GDF15.

Article Snippet: Long acting GDF15 , Novo Nordisk A/S , Cat#NNC-0247-0001-0880.

Techniques: Activity Assay

Journal: Cell reports

Article Title: Housing mice near vs. below thermoneutrality affects drug-induced weight loss but does not improve prediction of efficacy in humans

doi: 10.1016/j.celrep.2024.114501

Figure Lengend Snippet: Primer information

Article Snippet: Long acting GDF15 , Novo Nordisk A/S , Cat#NNC-0247-0001-0880.

Techniques:

Journal: Cell reports

Article Title: Housing mice near vs. below thermoneutrality affects drug-induced weight loss but does not improve prediction of efficacy in humans

doi: 10.1016/j.celrep.2024.114501

Figure Lengend Snippet:

Article Snippet: Long acting GDF15 , Novo Nordisk A/S , Cat#NNC-0247-0001-0880.

Techniques: Recombinant, Clinical Proteomics, Activity Assay, Expressing, Software